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Image Search Results
Journal: Cellular and Molecular Bioengineering
Article Title: A Stable Pep2-proapoptotic Peptide Inducing Apoptosis of Acute Myeloid Leukemia Cells by Down-Regulating EZH2
doi: 10.1007/s12195-019-00605-z
Figure Lengend Snippet: Identification of PP7 VLPs carrying Pep2-KLAK peptide. (a) Expression of 2PP7-Pep2-KLAK VLPs and 2PP7-Pep2 VLPs in the supernatant of BL21 (DE3) after sonication. The target proteins are marked by black arrows. (b) Purification chromatogram of 2PP7-Pep2-KLAK VLPs by gel filtration chromatography. The peaks of the target proteins are marked by black arrows. (c) Western blot of the pure VLPs. (d) Assembly of the PP7 coat protein dimer carrying Pep2-KLAK peptide into whole VLPs. The VLPs was observed using TEM at 60 kV and ×100,000 screen magnification. (e) Size analysis of VLPs by particle size analyzer.
Article Snippet: Then the supernatant was incubated with DNase I (20 U/mL) and RNase A (10 mg/mL) at 37 °C for 3 h, and purified by
Techniques: Expressing, Sonication, Purification, Filtration, Chromatography, Western Blot